Turf insect

Use an entomopathogenic nematode, Heterorhabditis bacteriophora to control long-horned beetle, Dorcadion pseudopreissi infesting turf. by Ganpati Jagdale

The application of an entomopathogenic nematode Heterorhabditis bacteriophora at the rate of 0.5 million infective juveniles per square meter can significantly reduce the population of Dorcadion pseudopreissi infesting turf grass (Lolium perenne) in the field (Susurluk et al. (2011). Read following papers for more information.

Susurluk, I.A., Kumral, N.A., Bilgili, U. and Acikgoz, E. 2011. Control of a new turf pest, Dorcadion pseudopreissi (Coleoptera: Cerambycidae), with the entomopathogenic nematode Heterorhabditis bacteriophora. Journal of Pest Science 84: 321-326.

Susurluk, I.A., Kumral, N.A., Peters, A., Bilgili, U. and Acikgoz, E. 2009.  Pathogenicity, reproduction and foraging behaviours of some entomopathogenic nematodes on a new turf pest, Dorcadion pseudopreissi (Coleoptera: Cerambycidae). Biocontrol Science and Technology 19: 585-594.

Entompathogenic nematodes used as biopesticides by Ganpati Jagdale

Entomopathogenic nematodes such as Steinernema carpocapsae and Heterorhabditis bacteriophora have been used to control white grubs that feed turfgrass in your yard. When applied in turf these nematodes search and infect white grubs. They infect grub insects through the natural openings and once inside they release symbiotic bacteria in the body cavity of grub. Bacteria multiply and kill insect within 48 hours of infection.

Damage caused by Japanese beetles by Ganpati Jagdale

Control of white grub Hoplia philanthus with entomopathogenic nematodes by Ganpati Jagdale

Efficacy of entomopathogenic nematodes including Heterorhabditis bacteriophora CLO51 strain, H. megidis VBM30 strain, H. indica, Steinernema scarabaei, S. feltiae, S. arenarium, S. carpocapsae Belgian strain, S. glaseri Belgian and NC strains was tested against larval pupal stages a white grub, Hoplia philanthus under laboratory and greenhouse conditions. Heterorhabditis bacteriophora, H. megidis and both strains of S. glaseri showed highest virulence against third stage larvae and pupae whereas Belgium strain of S. glaseri showed high virulence against second stage larvae of H. philanthus under laboratory conditions whereas H. bacteriophora, Belgium strains of S. glaseri and S. scarabaei showed high virulence to third stage than second stage larvae of white grubs under greenhouse conditions.


Ansari, M.A., Adhikari, B.N., Ali, F. and Moens, M. 2008. Susceptibility of Hoplia philanthus (Coleoptera: Scarabaeidae) larvae and pupae to entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae). Biological Control. 47: 315-321.

Efficacy of Heterorhabditis indica and Steinernema minuta against the Japanese beetle by Ganpati Jagdale

Efficacy of four new entomopathogenic nematode strains including Heterorhabditis indica strains MP17 and MP111, Heterorhabditis sp. strain MP68 and Steinernema  minuta strain MP10 from Thailand was tested against the second  instar larva of the Japanese beetle, Popillia japonica in laboratory bioassays. This study demonstrated that the strains of both H. indica and Heterorhabditis spp. were more efficacious against P. japonica that the strain of Steinernema  minuta. However, when comparisons were made among the strains of Heterorhabditis nematodes, MP111 strain of H. indica was the most efficacious against the second instar larva of Japanese beetle . Read following paper on the virulence of entomopathogenic nematodes from Thailand on Japanese beetle.

Maneesakorn, P., An, R., Grewal, P.S.and Chandrapatya, A. 2010. Virulence of our new strains of entomopathogenic nematodes from Thailand against second instar larva of the Japanese Beetle, Popillia japonica (Coleoptera: Scarabaeidae). Thai Journal of Agricultural Science.43: 61-66.

Control the annual bluegrass weevil, Listronotus maculicollis with entomopathogenic nematodes by Ganpati Jagdale

Recently, McGraw et al (2010) demonstrated that field application of three species of entomopathogenic nematodes (Steinernema carpocapsae, S. feltiae and Heterorhabditis bacteriophora) at rate of 2.5 billion nematodes/hectare reduced over 69% population of first generation late instars of the annual bluegrass weevil, Listronotus maculicollis. For more information on the interaction between entomopathogenic nematodes and the annual bluegrass weevil read following literature.

McGraw, B.A. and Koppenhofer A.M. 2008.  Evaluation of two endemic and five commercial entomopathogenic nematode species (Rhabditida : Heterorhabditidae and Steinernematidae) against annual bluegrass weevil (Coleoptera : Curculionidae) larvae and adults. Biological Control. 46: 467-475.

McGraw, B.A. and Koppenhofer A.M. 2009.  Population dynamics and interactions between endemic entomopathogenic nematodes and annual bluegrass weevil populations in golf course turfgrass. Applied Soil Ecology. 41: 77-89.

McGraw, B.A., Vittum, P.J., Cowles, R.S. and Koppenhofer A.M. 2010.  Field evaluation of entomopathogenic nematodes for the biological control of the annual bluegrass weevil, Listronotus maculicollis (Coleoptera: Curculionidae), in golf course turfgrass. Biocontrol Science and Technology. 20: 149-163.