Xenorhabdus spp-

Entomopathogenic nematodes kill their insect hosts within 24 hours by Ganpati Jagdale

Entomopathogenic Nematode Virulence- Nematode information It is well known fact that the infective juveniles of both Steinernema spp. and Heterorhabditis spp. enter their insect host through natural openings such as mouth, anus and spiracles and eventually reach in the insect body cavity.  As insects do not have a closed circulatory system like animals, their body cavity acts as an open circulatory system, which is filled with the blood that is technically called as hemolymph.

Read More

How entomopathogenic nematodes enter into host body- Nematode information? by Ganpati Jagdale

Entomopathogenic nematodes- Mode of Infection In the soil environment, infective juveniles of entomopathogenic nematodes (Figure 1.) are always searching for the insect hosts to infect, kill, feed and reproduce.  Once the infective juveniles of both Steinernematid (Steinernema spp.) and Heterorhabditid (Heterorhabditis spp.) nematodes locate any larval, pupal or adult stages of their insect host, they will rush to find any easy entry routes/points to enter into the insect host body. 

Read More

Why some insect-parasitic nematodes are called entomopathogenic nematodes? by Ganpati Jagdale

Entomopathogenic Nematodes- Nematode Information Insect-parasitic nematodes that belong to both Steinernematidae and Heterorhabditidae families are also called as entomopathogenic nematodes because they cause disease to their insect hosts with the help of mutualistically associated symbiotic bacterial pathogens.

Read More

Virulence Mechanisms of symbiotic bacteria Photorhabdus and Xenorhabdus spp by Ganpati Jagdale

Entomopathogenic nematodes and their symbiotic bacteria- Nematode Information

Molecular studies demonstrated that the closely related Photorhabdus, symbiotic bacteria of Heterorhabditis nematodes and Xenorhabdus, symbiotic bacteria of Steinernematid nematodes have developed totally different molecular strategies for the same objective of virulence to insects and symbiosis with the nematode.

These findings were presented by An, R. and Grewal, P.S. at the 50th annual meeting of the Society of Nematologists held in Corvallis, Oregon from July 17-20, 2011.

Mode of action of entomopathogenic nematodes by Ganpati Jagdale

When the infective juveniles of entomopathogenic nematodes are applied to the soil surface in the fields or thatch layer on golf courses, they start searching for their insect hosts. Once insect larva has been located, the nematode infective juveniles penetrate into the larval body cavity via natural openings such as mouth, anus and spiracles. Infective juveniles of Heterorhabditis nematodes can also enter through the intersegmental membranes of the grub cuticle. Once in the body cavity, infective juveniles release symbiotic bacteria (Xenorhabdus spp. for Steinernematidae and Photorhabdus spp. for Heterorhabditidae) from their gut in insect blood. In the blood, multiplying nematode-bacterium complex causes septicemia and kill their insect host usually within 48 h after infection. Nematodes feed on multiplying bacteria, mature into adults, reproduce and then emerge as infective juveniles from the host cadaver to seek new larvae in the soil.

Kill leafminers (Liriomyza spp.) with Entomopathogenic Nematodes by Ganpati Jagdale

  • Leafminers (Liriomyza spp.) are considered as economically important polyphagous pests of many indoor vegetable crops and flowering plants.
  • Vegetable host crops included beans, beet, carrots, celery, cucumbers, eggplants, lettuce, melons, onions, peas, peppers, potatoes, squash and tomatoes.
  • Flowering host plants included ageratum, aster, calendula, chrysanthemum, dahlia, gerbera, gypsophila, marigold, petunia, snapdragon, and zinnia.
  • Leafminer maggots generally feed on leaf parenchyma tissues by tunneling/mining between the upper and lower epidermal leaf surfaces.
  • Adults generally feed on sap exuding from the punctures caused by maggots during mining.
  • Infested leaves appear stippled due to the punctures made by leafminers while feeding, mining and oviposition especially at the leaf tip and along the leaf margins.
  • Widespread mining and stippling on the leaves generally decreases the level of photosynthesis in the plant leading towards the premature leaf drop reducing the amount of shade, which in turn causes sun scalding of fruits.
  • Injuries caused by maggots on the foliage also allow entry of bacterial and fungal disease causing pathogens.
  • Life cycle of leafminers contains four stages including egg, maggot, pupa and adult.
  • Life cycle can be completed within 15-21 days depending upon the host and temperature.
  • Adult females lay eggs in leaf tissues, eggs hatch within 2-3 days into maggots, hatched maggots starts feeding immediately and become mature within 3-4 days. Mature larvae eventually cut through the leaf epidermis and move to the soil for pupation and adults emerge within 3 weeks of pupation in the summer.
  • Although, chemical insecticides are generally used to protect foliage from injury caused by leafminers, but development of insecticide resistance among leafminer populations is a major problem.
  • Insecticides also are highly disruptive to naturally occurring biological control agents, particularly parasitoids.
  • Therefore, biological control agents including Bacillus thuringiensis var. thuringiensis (Bt), parasitic wasps (Diglyphus begina, D. intermedius, D. pulchripes and Chrysocharis parksi) and entomopathogenic nematodes (Heterorhabditis spp, Steinernema carpocapase and S. feltiae) have been considered as alternatives to chemical pesticides.
  • For successful control of leafminers, entomopathogenic nematodes can be easily applied in water suspension as spray application on plant foliage.
  • Entomopathogenice nematodes including S. carpocapase and S. feltiae when applied at the rate of 5.3 X 108 nematodes/ha can cause over 64% mortality of leafminers but need at least 92% relative humidity.

How Entomopathogenic Nematodes kill leafminers

  • When the infective juveniles are applied as spray to plant foliage, they enter the leaf mines through the leaf miner feeding punctures or exit holes made by the adults.
  • Once inside the mine the nematodes swim to find a leafminer maggot, nematodes then penetrate into the maggot body cavity via natural openings such as mouth, anus and spiracles.
  • Infective juveniles of Heterorhabditis also enter through the intersegmental members of the larval cuticle.
  • Once in the body cavity, infective juveniles release symbiotic bacteria (Xenorhabdus spp. for Steinernematidae and Photorhabdus spp. for Heterorhabditidae) from their gut in the maggot blood.
  • In the blood, multiplying nematode-bacterium complex causes septicemia and kills maggots usually within 48 h after infection.

For more information on the interaction between entomopathogenic nematodes and leafminers, please read following research and extension publications.

  • Hara, A.H., Kaya, H.K., Gaugler, R., Lebeck, L.M. and Mello, C.L. 1993. Entomopathogenic nematodes for biological control of the leafminer, Liriomyza trifolii (Dipt.: Agromyzidae).  Entomophaga 38, 359-369.
  • Head, J. and Walters, K.F.A. 2003.  Augmentation biological control utilising the entomopathogenic nematode, Steinernema feltiae, against the South American Leafminer, Liriomyza huidobrensis. Proceedings of the 1st International Symposium on Biological Control, (Hawaii, USA, 13-18 January 2002). USDA Forest Service, FHTET-03-05, 136-140.
  • Olthof, T.H.A. and Broadbent, A.B. 1992.  Evaluation of steinernematid nematodes for control of a leafminer, Liriomyza trifolii, in greenhouse chrysanthemums. Journal of Nematology 24, 612.
  • Tong-Xian Liu, Le Kang, K.M.Heinz, J.Trumble. 2008. Biological control of Liriomyza leafminers: progress and perspective. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 2009, 4, No. 004, 16 pp.
  • Williams, E.C. and Walters, K.F.A. 1994.  Nematode control of leafminers: Efficacy, temperature and timing.  Brighton Crop Protection Conference - Pests and Disease. 1079-1084.
  • Williams, E.C. and MacDonald, O.C., 1995.  Critical factors required by the nematode Steinernema feltiae for the control of the leafminers Liriomyza huidobrensis, Liriomyza bryoniae and Chromatomyia syngenesiae.  Annals of Applied Biology. 127, 329-341.
  • Williams, E.C. and Walters, K.F.A. 2000.  Foliar application of the entomopathogenic nematode Steinernema feltiae against leafminers on vegetables. Biocontrol Science and Technology 10, 61-70.

Kill Shore flies (Scatella stagnalis) with Entomopathogenic Nematodes by Ganpati Jagdale

  • The shore fly, Scatella stagnalis (Fallén) (Diptera: Ephydridae) is an important insect pest of greenhouse plants.
  • Larvae of these flies mainly feed on blue-green algae grown on the surface of plant growing media, walls, floors, benches, and pots.
  • But larvae can also cause a serious damage to tender plant tissues thus reducing quality and productivity of plants.
  • The adults are not considered as plant feeders but they are nuisance to people and disseminate pathogens such as Fusarium and Pythium from plant to plant as they disperse through the greenhouse.
  • Currently, most growers rely on chemicals that kill host plants such as blue-green algae to reduce the incidence of shore flies. However, this method has not been proved effective in reducing shore fly incidence.
  • Biological control agents including Bacillus thuringiensis var. thuringiensis (Bt) and entomopathogenic nematodes have been considered as alternatives to chemical pesticides.
  • For successful control of shore flies, entomopathogenic nematodes can be easily applied in water suspension as spray application to the surface of plant growing medium.
  • Entomopathogenice nematodes including Heterorhabditis megidis, Steinernema arenarium and Steinernema feltiae when applied at the rate of 50 nematodes/cm2 can cause 94- 100% mortality of shore flies.

How Entomopathogenic Nematodes kill Shore flies

  • When the infective juveniles are applied to the surface of plant growing substrate, they start searching for their hosts, in this case shore fly larvae.
  • Once a larva has been located, the nematode infective juveniles penetrate into the larval body cavity via natural openings such as mouth, anus and spiracles.
  • Infective juveniles of Heterorhabditis spp also enter through the intersegmental members of the larval cuticle.
  • Once in the body cavity, infective juveniles release symbiotic bacteria (Xenorhabdus spp. for Steinernematidae and Photorhabdus spp. for Heterorhabditidae) from their gut in the larval blood.
  • In the blood, multiplying nematode-bacterium complex causes septicemia and kills shore fly larvae usually within 48 h after infection.
  • Nematodes feed on multiplying bacteria, mature into adults, reproduce and then emerge as infective juveniles from the cadaver to seek new larvae in the potting medium/soil.

For more information on the interaction between entomopathogenic nematodes and leafminers, please read following research and extension publications.

  • Foote, B.A. 1977.  Utilization of blue-breen algae by larvae of shore flies. Environmental Entomology 6, 812-814.
  • Goldberg, N.P. and Stanghellini, M.E. 1990.  Ingestion-egestion and aerial transmission of Pythium aphanidermatum by shore flies (Ephydrinae: Scatella stagnalis). Phytopathology 80, 1244-1246.
  • Lindquist, R., Buxton, J. and Piatkowski, J. 1994.  Biological control of sciarid flies and shore flies in glasshouses. Brighton Crop Protection Conference, Pests and Diseases, BCPC Publications 3, 1067-1072.
  • Morton, A., Garcia del Pino, F., 2007.  Susceptibility of shore fly Scatella stagnalis to five entomopathogenic nematode strains in bioassays. Biocontrol 52: 533-545.
  • Morton, A. and Garcia del Pino, F. 2003. Potential of entomopathogenic nematodes for the control of shore flies (Scatella stagnalis). Growing Biocontrol Markets Challenge Research and Development. 9th European Meeting IOBC/WPRS Working Group "Insect Pathogens and Entomopathogenic Nematodes", Abstracts, 67.
  • Vanninen, I., Koskula, H. 2000. Biological control of the shore fly (Scatella tenuicosta) with steinernematid nematodes and Bacillus thuringiensis var. thuringiensis in peat and rockwool. Biocontrol Sci. Technol.. 13: 47-63.
  • Zack, R.S. and Foote, B.A. 1978.  Utilization of algal monoculture by larvae of Scatella stagnalis. Environmental Entomology 7, 509-511.

Symbiotic bacteria of Steinernematid nematodes- Xenorhabdus species by Ganpati Jagdale

  1. Steinernema abbasi- undescribed
  2. S. aciari- undescribed
  3. S. affine-Xenorhabdus bovienii
  4. S. akhursti- undescribed
  5. S. anatoliense- undescribed
  6. S. apuliae- undescribed
  7. S. arenarium- X. kozodoii
  8. S. ashiuense- undescribed
  9. S. asiaticum- undescribed
  10. S. australe- X. magdalenensis
  11. S. backanense- undescribed
  12. S. beddingi- undescribed
  13. S. bicornutum- X. budapestensis
  14. S. carpocapsae- X. nematophila
  15. S. caudatum- undescribed
  16. S. ceratophorum- undescribed
  17. S. cholashanense- undescribed
  18. S. cubanum- X. poinarii
  19. S. cumgarense- undescribed
  20. S. diaprepesi- undescribed
  21. S. eapokense- undescribed
  22. S. feltiae- X. bovienii
  23. S. glaseri- X. poinarii
  24. S. guangdongense- undescribed
  25. S. hebeiense- undescribed
  26. S. hermaphroditum- undescribed
  27. S. intermedium - X. bovienii
  28. S. jollieti-undescribed
  29. S. karii- undescribed
  30. S. khoisanae- undescribed
  31. S. kraussei- X. bovienii
  32. S. kushidai- X. japonica
  33. S. leizhouense- undescribed
  34. S. litorale- undescribed
  35. S. loci- undescribed
  36. S. longicaudum- undescribed
  37. S. monticolum- undescribed
  38. S. neocurtillae- undescribed
  39. S. oregonense- undescribed
  40. S. pakistanense- undescribed
  41. S. puertoricense- X. romanii
  42. S. rarum- X. szentirmaii
  43. S. riobrave- Xenorhabdus sp
  44. S. ritteri- Xenorhabdus sp
  45. S. robustispiculum- undescribed
  46. S. sangi- undescribed
  47. S. sasonense- undescribed
  48. S. scapterisci- X. innexi
  49. S. scarabaei- X. koppenhoeferi
  50. S. serratum- X. ehlersii
  51. S. siamkayai- X. stockiae
  52. S. sichuanense- X. bovienii
  53. S. silvaticum- undescribed
  54. S. tami- Xenorhabdus sp
  55. S. texanum- undescribed
  56. S. thanhi- undescribed
  57. S. thermophilum- X. indica
  58. S. websteri- undescribed
  59. S. weiseri- undescribed
  60. S. yirgalemense- undescribed

Visit this blog again for new updates (if any) on Steinernema species and their associated bacterial species

Symbiotic bacterial genus, Xenorhabdus Thomas and Poinar 1979 by Ganpati Jagdale

known species of symbiotic bacterial genus Xenorhabdus Thomas and Poinar 1979 associated with a nematode genus Steinernema. Identification based on colony morphology and molecular techniques

  1. Xenorhabdus beddingii (Akhurst 1986) Akhurst and Boemare 1993
  2. X. bovienii (Akhurst 1983) Akhurst and Boemare 1993
  3. X. budapestensis Lengyel, Lang, Fodor, Szállás, Schumann, Stackebrandt, 2005
  4. X. cabanillasii Tailliez, Pagès, Ginibre & Boemare, 2006
  5. X. doucetiae Tailliez, Pagès, Ginibre & Boemare, 2006
  6. X. ehlersii Lengyel, Lang, Fodor, Szállás, Schumann, Stackebrandt, 2005
  7. X. griffiniae Tailliez, Pagès, Ginibre & Boemare, 2006
  8. X. hominickii Tailliez, Pagès, Ginibre & Boemare, 2006
  9. X. indica Somvanshi, Lang, Ganguly, Swiderski, Saxena, & Stackebrandt 2006
  10. X. innexi Lengyel, Lang, Fodor, Szállás, Schumann, Stackebrandt, 2005
  11. X. japonica Nishimura et al. 1995
  12. X. koppenhoeferi Tailliez, Pagès, Ginibre & Boemare, 2006
  13. X. kozodoii Tailliez, Pagès, Ginibre & Boemare, 2006
  14. X. magdalenensis, Tailliez, Pages, Edgington, Tymo, & Buddie, 2012
  15. X. mauleonii Tailliez, Pagès, Ginibre & Boemare, 2006
  16. X. miraniensis Tailliez, Pagès, Ginibre & Boemare, 2006
  17. X. nematophila (Poinar and Thomas 1965) Thomas and Poinar 1979
  18. X. poinarii (Akhurst 1983) Akhurst and Boemare 1993
  19. X. romanii Tailliez, Pagès, Ginibre & Boemare, 2006
  20. X. stockiae Tailliez, Pagès, Ginibre & Boemare, 2006
  21. X. szentirmaii Lengyel, Lang, Fodor, Szállás, Schumann, Stackebrandt, 2005

Visit this blog again for new updates (if any) on Xenorhabdus species