Molecular technique

Quantitative real-time PCR techniques for detecting and quantifying entomopathogenic nematodes from the soil samples by Ganpati Jagdale

Recently, a quantitative real-time PCR (qPCR) technique has been developed by Campos-Herrera et al (2011) for detecting and quantifying entomopathogenic nematodes including Steinernema diaprepesi, Steinernema riobrave, Heterorhabditis indica, Heterorhabditis zealandica, Heterorhabditis floridensis and an undescribed species in the S. glaseri group from soil samples. Read following paper for a detail protocol of quantitative real-time PCR (qPCR) technique

Campos-Herrera, R., Johnson, E. G, El-Borai, F. E., Stuart, R. J., Graham, J. H. and Duncan, L. W.2011. Long-term stability of entomopathogenic nematode spatial patterns in soil as measured by sentinel insects and real-time PCR. Annals of Applied Biology    158: 55-68.