Recently a survey was conducted to study the occurrence and distribution of entomopathogenic nematodes in Nepal. Although a total of 276 soil samples were collected from various habitats, entomopathogenic nematode were found only in 29 samples. Nematodes were isolates using the Galleria-baiting technique (Bedding and Akhurst,1975). Both heterorhabditid and steinernematid nematodes were identified at their species level using both molecular and morphological techniques. In this survey, the occurrence of only one species of heterorhabditids including Heterorhabditis indica and four described species of steinernematids such as Steinernema abbasi, S. cholashanense, S. feltiae and S. siamkayai were reported for the first time in Nepal (Khatri-Chhetri et al., 2010). Read following literature for more information
Bedding, R.A. and R.J. Akhurst. 1975. A simple technique for detection of insect parasitic rhabditid nematodes in soil. Nematologica. 21: 109-110.
Khatri-Chhetri, H.B., Waeyenberge, L., Manandhar, H.K. and Moens, M. 2010. Natural occurrence and distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in Nepal. Journal of Invertebrate Pathology. 103: 74-78.