New record of entomopathogenic nematode species

A new beneficial nematode Steinernema sacchari from South Africa by Ganpati Jagdale

A new beneficial entomopathogenic nematode collected from a sugarcane field located in the KwaZulu-Natal province of South Africa was named as Steinernema sacchari.  

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A new species of entomopathogenic Steinernema nematodes by Ganpati Jagdale

A new species of entomopathogenic Steinernema nematode that isolated from southwest Bohemia, Czech Republic was identified and named as Steinernema poinari sp. n. (Nematoda : Steinernematidae) using both morphological and molecular techniques (Mráček et al., 2014). This new species was recovered from soil using Galleria baiting technique described by Bedding and Akhurst (1975).

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A new Steinernematid nematode species from India by Ganpati Jagdale

A new Steinernematid nematode species isolated from central part of India was named as Steinernema dharanaii sp. n. (Nematoda : Steinernematidae) by Kulkarni et al (2012) using both morphological and molecular techniques based on ITS rDNA.  These researchers found that this new species was closely associated with 'glaseri-group' of Steinernema spp. but its infective juveniles (Fig. 1), males and females had distinct morphological characteristics.

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Steinernema costaricense the beneficial Nematode found in Missouri by Ganpati Jagdale

The measurement of both the morphological and molecular characteristics showed that the newly isolated beneficial nematode from Missouri, USA is closely related to the entomopathogenic nematode Steinernema costaricense, which was originally isolated from Costa Rica in 2007 (Uribe-Lorio et al., 2007).

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A new entomopathogenic Stienernematid nematode from Ethiopia by Ganpati Jagdale

 Steinernema ethiopiense- Nematodeinformation

Using Galleria mellonella baiting technique (Bedding and Akhurst, 1975), an entomopthogenic nematode isolated from Ethiopia was identified as Stienernematid nematode. Based on both morphological and molecular characteristics, this entomopathogenic nematodes was considered as a new species and named as Steinernema ethiopiense (Tamiru et al., 2012).

For detail information on its morphological and molecular characteristics of this new species and its comparisons with other known species of Steinernematid nematodes read following literature.

Literature:

Bedding, R.A. and R.J. Akhurst. 1975. A simple technique for detection of insect parasitic rhabditid nematodes in soil. Nematologica. 21: 109-110.

Tamiru, T., Waeyenberge, L., Hailu, T., Ehlers, R.-U., Půža, V., Mráček, Z. 2012.  Steinernema ethiopiense sp. n. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Ethiopia. Nematology 14: 741- 757.

A new entomopathogenic Stienernematid nematode from China by Ganpati Jagdale

Steinernema xinbinense- Nematodeinformation

Using Galleria mellonella baiting technique (Bedding and Akhurst, 1975), a new species of entomopthogenic nematode collected from Liaoning province of North China was identified as Stienernematid nematode and based on both morphological and molecular characteristics it was named as Steinernema xinbinense (Ma et al., 2012).

For detail information on the morphological and molecular characteristics of this new species read following literature.

Literature:

Bedding, R.A. and R.J. Akhurst. 1975. A simple technique for detection of insect parasitic rhabditid nematodes in soil. Nematologica. 21: 109-110.

Ma, J., Chen, S., De Clercq, P., Waeyenberge, L., Han, R. and Moens, M. 2012. A new entomopathogenic nematode, Steinernema xinbinense n. sp. (Nematoda: Steinernematidae), from north China. Nematology 14: 723-739

A new entomopathogenic heterorhabditid nematode from South Africa by Ganpati Jagdale

Heterorhabditis noenieputensis

Based on both morphological and molecular characteristics, a new species of entomopthogenic nematode collected from a Citrus orchard was identified as Heterorhabditid nematode and named as Heterorhabditis noenieputensis. For detail information on its morphological and molecular characteristics and their comparisons with other closely related species of heterorhabditid nematodes read following literature.

Literature:

Malan, A.P., Knoetze, R. and Tiedt, L. 2012. Heterorhabditis noenieputensis n. sp. (Rhabditida: Heterorhabditidae), a new entomopathogenic nematode from South Africa. Journal of Helminthology 12:1-13.

A new entomopathogenic Heterorhabdtid nematode from China by Ganpati Jagdale

Heterorhabditid Nematode: Heterorhabditis beicherriana

Based on both morphological and molecular characteristics an entomopathogenic nematode isolated from an orchard located near Beijing, China was considered as a new species Heterorhabditid nematode and named as Heterorhabditis beicherriana (Li et al., 2012). For more information on molecular technique read following literature.

Literature:

Li, X.Y., Liu, Q.Z., Nermut, J., Puza, V. and Mracek, Z. 2012. Heterorhabditis beicherriana n. sp (Nematoda: Heterorhabditidae), a new entomopathogenic nematode from the Shunyi district of Beijing, China. Zootaxa  Issue: 3569: 25-40.  

New entomopathogenic nematode Steinernema australe from an island Isla Magdalena by Ganpati Jagdale

New entomopathogenic nematode and its symbiotic bacteria

Based on both the morphological and molecular characteristics, an entomopathogenic Steinernematid nematode isolated from a soil sample collected from Chilean island, Isla Magdalena has been identified as a new species, Steinernema australe (Edgington et al., 2009). This nematode is also symbiotically associated with symbiotic bacteria called Xenorhabdus magdalenensis, which was identified using 16S rRNA gene sequence similarities and a multigene approach (Tailliez et al., 2012).

Literature

Edgington, S., Buddie, A.G., Tymo, L., Hunt, D.J., Nguyen, K.B., France, A.I., Merino, L.M. and Moore, D. 2009. Steinernema australe n. sp. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode from Isla Magdalena, Chile. Nematology 11: 699-717.

Tailliez, P., Pages, S., Edgington, S., Tymo, L.M. and Buddie, A.G. 2012. Description of Xenorhabdus magdalenensis sp nov., the symbiotic bacterium associated with Steinernema australe. International Journal of Systematic and Evolutionary Microbiology 62: 1761-1765.

A report of new entomopathogenic nematode species from Florida by Ganpati Jagdale

Steinernema phyllophagae- Nematode Information

Last year a new species of entomopathogenic nematode was isolated by Nguyen and Buss (2011) from a white grub (Phyllophaga sp.) and based on morphological and molecular characteristics, it was named as Steinernema phyllophagae.

Literature

Nguyen, K.B., and Buss, E.A. 2011. Steinernema phyllophagae n. sp (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Florida, USA. Nematology 13: 425-442.

A new entomopathogenic nematode species from South Africa by Ganpati Jagdale

An entomopathogenic nematode, Steinernema citrae- Nematode Information Galleria-baiting technique (Bedding and Akhurst, 1975), was used to isolate an entomopathogenic nematode from the soil collected from a citrus orchard in South Africa. Based on molecular characteristics, this new nematode was named as Steinernema citrae that  found to be closely related Stienernema feltiae group.

For detail information read following original paper

Bedding, R.A. and R.J. Akhurst. 1975. A simple technique for detection of insect parasitic rhabditid nematodes in soil. Nematologica. 21: 109-110.

Stokwe, N.F., Malan, A.P., Nguyen, K.B., Knoetze, R. and Tiedt, L. 2011. Steinernema citrae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa. Nematology 13: 569-587.

A new entomopathogenic nematode Heterorhabditis atacamensis from Chile by Ganpati Jagdale

An entomopathogenic nematode, Heterorhabditis atacamensis- Nematode Information New entomopathogenic nematode species was found in the soil collected from Atacama Desert in Chile and was named after Atacama Desert as Heterorhabditis atacamensis. I like the way nematode taxonomists (Edgington et al., 2011) used individual morphological characteristics to differentiate this new species from other morphologically similar species of entomopathogenic nematodes. For example, these researchers showed that the H. atacamensis differed from H. marelatus, H. downesi and H. amazonensis based on position of hemizonid (a nematode sensory organ), position of excretory pore and female tail terminus shape, and number and position of genital papillae, respectively.  Using molecular techniques, Edgington et al. (2011) were also able to distinguish H. atacamensis from closely related entomopathogenic nematode species, H. safricana.

 Research Paper

Edgington, S., Buddie, A. G., Moore, D., France, A., Merino, L. and Hunt, D. J. 2011. Heterorhabditis atacamensis n. sp (Nematoda: Heterorhabditidae), a new entomopathogenic nematode from the Atacama Desert, Chile. Journal of Helminthology 85: 381-394.

New entomopathogenic nematode, Oscheius carolinensis found in vermicompost by Ganpati Jagdale

New entomopathogenic nematode, Oscheius carolinensis- Nematode Information Based on morphological and molecular characteristics, the nematode isolated from vermicompost using Galleria bait method (Bedding and Akhurst, 1975) has been described as a new entomopathogenic nematode species, Oscheius carolinensis (Ye et al., 2010).  This nematode is also pathogenic to cabbage butterfly (Pieris rapae) and mealworms (Tenebrio molitor).

 Literature

Bedding, R.A. and R.J. Akhurst. 1975. A simple technique for detection of insect parasitic rhabditid nematodes in soil. Nematologica. 21: 109-110.

Ye, W., Torres-Barragan, A. and Cardoza, Y.J. 2010. Oscheius carolinensis n. sp. (Nematoda: Rhabditidae), a potential entomopathogenic nematode from vermicompost. Nematology 12: 121-135.

Occurrence of entomopathogenic nematodes in Egypt: Nematode Information by Ganpati Jagdale

Entomopathogenic nematodes from Egypt

The occurrence and distribution of entomopathogenic nematodes including Heterorhabditis indica, Steinernema abbasi and Steinernema carpocapsae have been reported from four geographical regions (Northern, Middle, Southern and Sinai Peninsula) of Egypt.

These findings were presented by Abu-Shady, N.M., Shamseldean, M.M., Abd-Elbary, N.A. and Stock, S.P. at the 50th annual meeting of the Society of Nematologists held in Corvallis, Oregon from July 17-20, 2011.

Entomopathogenic nematode Steinernema siamkayai from Thailand by Ganpati Jagdale

An entomopathogenic nematode from Thailand:Nematode Information Based on the morphological and molecular studies, entomopathogenic nematode, Steinernema siamkayai was reported as a new species from Thailand in 1998.

Publication:

  1. Stock, S.P., Somsook, V. and Reid, A.P. 1998. Steinernema siamkayai n. sp. (Rhabditida: Steinernematidae), an entomopathogenic nematode from Thailand. Systematic Parasitology 41: 105-113.

Entomopathogenic nematode Steinernema siamkayai reported from India- Nematode information by Ganpati Jagdale

A warm-adapted entomopathogenic nematode Steinernema siamkayai Tiruchirappalli strain can cause 45-100% larval mortality of various insect species including Galleria mellonellaSpodoptera exiguaCeratitis capitataCydia splendana and Tenebrio molitor when tested under laboratory conditions at temperatures between 15- 37C (Raja et al., 2011).

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Entomopathogenic nematodes for the biological control of False codling moth- Nematode information by Ganpati Jagdale

Entomopathogenic nematodes and False codling moth
  • A presence of entomopathogenic nematode species including Steinernema khoisanae, Steinernema yirgalemense, Steinernema citrae, Heterorhabditis bacteriophora and Heterorhabditis zealandica have been reported in citrus orchards in the Western Cape, Eastern Cape and Mpumalanga provinces of South Africa (Malan et al., 2011).
  • All the above nematode species have showed a very high virulence against false codling moth, Thaumatotibia leucotreta an economically important pest of citrus in South Africa.  For example, S. yirgalemense can cause over 74% mortality of both larval and pupal mortality of false codling moth when applied at the rate of 50-200 infective juveniles/ larval or pupal stages of false codling moth.
  • Two entomopathogenic nematode species including S. yirgalemense and S. citrae were reported for the first time from South Africa (Malan et al., 2011).

Read following papers on entomopathogenic nematodes from South Africa

de Waal, J.Y., Malan, A.P. and Addison, M.F. 2011.  Evaluating mulches together with Heterorhabditis zealandica (Rhabditida: Heterorhabditidae) for the control of diapausing codling moth larvae, Cydia pomonella (L.) (Lepidoptera: Tortricidae).  Biocontrol Science and Technology 21: 255-270.

de Waal, J.Y., Malan, A.P., Levings, J. and Addison, M.F. 2010.  Key elements in the successful control of diapausing codling moth, Cydia pomonella (Lepidoptera: Tortricidae) in wooden fruit bins with a South African isolate of Heterorhabditis zealandica (Rhabditida: Heterorhabditidae). Biocontrol Science and Technology. 20: 489-502.

Hatting, J., Stock, S.P. and Hazir, S.  2009. Diversity and distribution of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in South Africa.  Journal of Invertebrate Pathology 102: 120-128.

Malan, A.P., Knoetze, R. and Moore, S.D.  2011.  Isolation and identification of entomopathogenic nematodes from citrus orchards in South Africa and their biocontrol potential against false codling moth. Journal of Invertebrate Pathology 108: 115-125.

Malan, A.P., Nguyen, K. B. and Addison, M. F. 2006.  Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from the southwestern parts of South Africa. African Plant Protection 12: 65-69.

Malan, A.P., Nguyen, K.B., de Waal, J.Y. and Tiedt, L. 2008. Heterorhabditis safricana n. sp (Rhabditida : Heterorhabditidae), a new entomopathogenic nematode from South Africa. Nematology 10: 381-396.

First record of entomopathogenic nematodes in Labanon by Ganpati Jagdale

A presence of entomopathogenic nematode species including Heterorhabditis bacteriophora and Steinernema feltiae has been reported for the first time in Lebanon (Noujeim et al., 2011). Read following paper for survey methods

Noujeim, E., Khater, C., Pages, S., Ogier, J.C., Tailliez, P., Hamze, M. and Thaler, O. 2011. The first record of entomopathogenic nematodes (Rhabiditiae: Steinernematidae and Heterorhabditidae) in natural ecosystems in Lebanon: A biogeographic approach in the Mediterranean region. Journal of Invertebrate Pathology 107: 82-85.

A new entomopathogenic nematode species, Heterorhabditis sonorensis from Mexico by Ganpati Jagdale

Recently, a new entomopathogenic nematode species Heterorhabditis sonorensis has been reported from Mexico.  This nematode was recovered from nymphal stages of cicada Diceroprocta ornea collected from an asparagus field. Please read following research paper on techniques used for the identification of this new entomopathogenic nematode species.

Stock, S.P., Rivera-Orduno, B. and Flores-Lara, Y. 2009. Heterorhabditis sonorensis n. sp (Nematoda: Heterorhabditidae), a natural pathogen of the seasonal cicada Diceroprocta ornea (Walker) (Homoptera: Cicadidae) in the Sonoran desert. Journal of Invertebrate Pathology. 100: 175-184.